g l brain heart infusion Search Results


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Becton Dickinson brain heart infusion 37 g/liter
Prophage-free V. natriegens strain outperforms the wild type in a competitive growth experiment. Equal ratios of the indicated strains were cultivated in <t>BHIN</t> with 0.5%, 1.5%, or 2.5% <t>(wt/vol)</t> <t>NaCl</t> in a repetitive batch approach. Twice a day, stationary-phase cells were transferred to fresh medium (dilution, 1:200). The experiment was performed for 12 cycles (∼90 generations) in biological triplicates (A, B, and C). Culture composition was analyzed by qPCR. Shown is the composition of the initial cultures (cycle 1, ∼1:1) were compared with cells from the last batch (cycle 12).
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Prophage-free V. natriegens strain outperforms the wild type in a competitive growth experiment. Equal ratios of the indicated strains were cultivated in <t>BHIN</t> with 0.5%, 1.5%, or 2.5% <t>(wt/vol)</t> <t>NaCl</t> in a repetitive batch approach. Twice a day, stationary-phase cells were transferred to fresh medium (dilution, 1:200). The experiment was performed for 12 cycles (∼90 generations) in biological triplicates (A, B, and C). Culture composition was analyzed by qPCR. Shown is the composition of the initial cultures (cycle 1, ∼1:1) were compared with cells from the last batch (cycle 12).
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Merck KGaA 5% brain heart infusion (bhi) (37 g l 1)
Prophage-free V. natriegens strain outperforms the wild type in a competitive growth experiment. Equal ratios of the indicated strains were cultivated in <t>BHIN</t> with 0.5%, 1.5%, or 2.5% <t>(wt/vol)</t> <t>NaCl</t> in a repetitive batch approach. Twice a day, stationary-phase cells were transferred to fresh medium (dilution, 1:200). The experiment was performed for 12 cycles (∼90 generations) in biological triplicates (A, B, and C). Culture composition was analyzed by qPCR. Shown is the composition of the initial cultures (cycle 1, ∼1:1) were compared with cells from the last batch (cycle 12).
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Becton Dickinson blood agar plates consisting of 52 g/l brain heart infusion agar medium
Prophage-free V. natriegens strain outperforms the wild type in a competitive growth experiment. Equal ratios of the indicated strains were cultivated in <t>BHIN</t> with 0.5%, 1.5%, or 2.5% <t>(wt/vol)</t> <t>NaCl</t> in a repetitive batch approach. Twice a day, stationary-phase cells were transferred to fresh medium (dilution, 1:200). The experiment was performed for 12 cycles (∼90 generations) in biological triplicates (A, B, and C). Culture composition was analyzed by qPCR. Shown is the composition of the initial cultures (cycle 1, ∼1:1) were compared with cells from the last batch (cycle 12).
Blood Agar Plates Consisting Of 52 G/L Brain Heart Infusion Agar Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Prophage-free V. natriegens strain outperforms the wild type in a competitive growth experiment. Equal ratios of the indicated strains were cultivated in BHIN with 0.5%, 1.5%, or 2.5% (wt/vol) NaCl in a repetitive batch approach. Twice a day, stationary-phase cells were transferred to fresh medium (dilution, 1:200). The experiment was performed for 12 cycles (∼90 generations) in biological triplicates (A, B, and C). Culture composition was analyzed by qPCR. Shown is the composition of the initial cultures (cycle 1, ∼1:1) were compared with cells from the last batch (cycle 12).

Journal: Applied and Environmental Microbiology

Article Title: Generation of a Prophage-Free Variant of the Fast-Growing Bacterium Vibrio natriegens

doi: 10.1128/AEM.00853-19

Figure Lengend Snippet: Prophage-free V. natriegens strain outperforms the wild type in a competitive growth experiment. Equal ratios of the indicated strains were cultivated in BHIN with 0.5%, 1.5%, or 2.5% (wt/vol) NaCl in a repetitive batch approach. Twice a day, stationary-phase cells were transferred to fresh medium (dilution, 1:200). The experiment was performed for 12 cycles (∼90 generations) in biological triplicates (A, B, and C). Culture composition was analyzed by qPCR. Shown is the composition of the initial cultures (cycle 1, ∼1:1) were compared with cells from the last batch (cycle 12).

Article Snippet: V. natriegens strains were cultivated in LBN (LB medium with 15 g/liter NaCl in total), BHIN (37 g/liter brain heart infusion [Becton, Dickinson, Franklin Lakes, NJ] plus 15 g/liter NaCl) or in VN medium ( ) [per liter, 21 g 3-( N -morpholino)propanesulfonic acid (MOPS), 5 g (NH 4 ) 2 SO 4 , 15 g NaCl, 1 g, KH 2 PO 4 , 1 g K 2 HPO 4 , 0.25 g MgSO 4 , 0.01 g CaCl 2 , 16.4 mg FeSO 4 × 7 H 2 O, 10 mg MnSO 4 × H 2 O, 0.3 mg CuSO 4 × 5 H 2 O, 1 mg ZnSO 4 × 7 H 2 O, 0.02 mg NiCl 2 × 6 H 2 O, pH 7.5].

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